Bruker’s timsTOF Ultra mass spectrometer sets new sensitivity and precision benchmarks

Bruker Corporation claims its newest addition, the timsTOF Ultra mass spectrometer, is poised to revolutionise proteomics with its advanced 4D-Proteomics timsTOF platform.

The timsTOF Ultra mass spectrometer features a new Captive Spray Ionization (CSI) Ultra ion source, 4th-generation TIMS (trapped ion mobility separation) XR cell, and a 14-bit digitiser. With unmatched sensitivity, the timsTOF Ultra can identify over 55,000 peptides mapping to 5,000 protein groups at a single-cell level, setting a new benchmark in sensitivity and quantitation performance. This is a significant advancement for single-cell proteomics and lipidomics; immunopeptidomics; phosphoproteomics; PTM analysis; and protein-protein interaction studies.

VistaScan software and advanced midia-PASEF mode

Bruker is also introducing VistaScan software for synchronizing the TIMS ramp with the quadrupole scan to benefit from ion mobility information at scale. This dynamic linked-ramp supports the novel midia-PASEF mode, which transforms data into data-dependent acquisition (dda)-like spectra. Professor Dr. Stefan Tenzer, a collaborator on this development, highlights its immense potential for immunopeptidomics and phosphoproteomics. Bruker has exclusively licensed midia-PASEF from the University Medical Center of the Johannes-Gutenberg University Mainz and aims to release it as a product in 2023.

Further advances in captive spray ionization and emitter design

The timsTOF Ultra’s CSI Ultra ion source, integral for the highest nanoflow sensitivity, has been refined further, focusing the vortex gas at the nanospray tip to improve ion transmission. Meanwhile, Bruker’s successful VIP-HESI ion source now comes equipped with new microFlow emitter capabilities. This advancement enables efficient ionisation at microflow and analytical LC flow rates, supporting the timsTOF platform’s ultra-high sensitivity, robustness and speed.

Real-Time QC capability in Bruker ProteoScape software with Biognosys iRT kit

Bruker introduces 4D-Proteomics ProteoScape software for GPU-powered real-time analysis with new capabilities for quality control (QC) using the Biognosys iRT kit. This software is crucial for system performance monitoring in real-time, which preserves precious clinical samples and ensures sensitive, robust, traceable and analytically high-quality results. The Bruker ProteoScape software also integrates third-party tools for accurate de novo sequencing for immunopeptidomics and meta-proteomics.

Integrated multiomic workflow in SCiLS Lab and OligoQuest 2.0 software updates

SCiLS Lab 2024 now includes MetaboScape annotation for lipid and metabolite annotation and enhances CCS-enabled 4D feature finding to speed up multiomic spatial tissue biology. Furthermore, OligoQuest 2.0 now enables sequence confirmation of full-length products and side products by intact mass and MS/MS analysis. This new capability allows for the customisation of the oligonucleotide “alphabet” for denoting standard or customised modifications, which the company claims offer unprecedented insights into complex biological systems.

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